antimicrobial activity of the amniotic fluid in Egyptian women

ABSTRACT

The antibacterial activity of 10 samples of amniotic fluid [AF] against Staph. aureus, E. coli and B. haemolytic streptococci was studied by adding 0.2 ml of a suitable dilution of log phase organisms in trypticase soy broth to 1.8 ml AF to give a final concentration of 2 x 103 organisms/ml, and then incubation at 37C for 24 hours. Nine of the 10 samples showed antibacterial activity to at least one organism. Seven samples were inhibitory to Staph. aureus [3 bactericidal, 4 bacteriostatic]. Six samples were inhibitory to E. coli [3 bactericidal, 3 bacteriostatic] and only 4 samples were inhibitory to beta-hemolytic streptococci [all bacteriostatic]