Some biochemical investigations and histopathological changes of liver in albino rats experimentally treated with nalbuphine [Nubain]

ABSTRACT

Forty adult albino rats were used in this investigation. They were divided into four groups each of ten. The first group was used as control. The second, third and fourth group were injected with Nalbuphine [Nubain] for 30, 60 and 90 days respectively. The injection was done intramuscularly and daily with 1 mg/lg B.W. At the end of experiment blood samples were collected from each group and the sera were obtained for immediate determination of aspartate amino transferase [ASAT], alanine aminotransferase [ALAT], alkaline phosphatase [ALP], seruloplasmin, total proteins and its fractions. Specimens from liver were processed for histopathological examination. There was a significant decrease in the amount of total proteins and albumins with a significant increase in beta and gamma globulins in all treated group, while the alpha 1 globulins were significantly decreased in the last two groups. A significant increase in ASAT, ALAT and ALP were detected in the sera of rats treated for 60 and 90 days i.e. the third and fourth groups. The ceruloplasmin was significantly decreased in the sera of all treated rats. The histopathological changes of liver revealed congested central veins, leucoytic infiltration and hydropic degeneration in rats treated for 30 days while those treated for 60 and 90 days showed coagulative necrosis