use of non-radioactive probes in the detection of human immunoglobulin genes

ABSTRACT

Contrarily to the radioactive probes, the nonradioactive ones are very stable and do not present any danger to health. This raised the need of the present work which aimed at preparing nonradioactive DNA probes which are then used to detect human immunoglobulin alpha genes. For this, DNA probes recognized by a specific antibody were prepared bearing the alkaline phosphatase [Digoxigenin-11-dUTP method] and then this probe was used for the detection of the cloned and genomic human immunoglobulin alpha genes. The results obtained, for the first time in this context, are in good correlation with the radioactive labeling tests. Thus, it is possible now to use nonradioactive technique to study the human immunoglobulin polymorphisms and the genetic diseases by restriction fragment length polymorphism [RFLP] without the known hazards of radioactivity