Inhibition of gingival fibroblasts proliferation in patients with juvenile periodontitis

ABSTRACT

Inhibition of gingival fibroblasts proliferation in vitro was investigated in patients with juvenile periodontitis. Twenty patients of both sexes, ranging in ago from 18-22 years and suffering from juvenile periodontitis; were selected to share in this study. A control group of similar number, age sex and with clinically healthy gingivae was also selected to participate in the study. For all patients, gingival biopsies were obtained and tissue cultures were accomplished for gingival fibroblasts. Subgingival dental plaque was collected from juvenile periodontitis patients; for the isolation and preparation of different strains of Aa sonicates. Different dilutions of Aa soni-cates were added to gingival fibroblasts cultures of both groups for cytotoxicity testing. Detachment of gingival fibroblasts were also tested; and proved not to be a precise method of assessing cytotoxicity. Aa-sonicates of different strains contained a heat stable cytotoxic components which inhibited proliferation of gingival fibroblasts in a dose-dependent fashion; as measured by cell viability. The inhibition was increasingly apparent in juvenile periodontitis group as compared to contral group. It could be concluded that, the high prevalence of Aa in the subgingival plaque of juvenile periodontogic agent in this entity of periodontal disease. Moreover, in juvenile periodontitis; inhibition of gingival fibroblasts proliferation with release of cytotoxic components was apparently detrimental in bing confined to Aa