Estradiol and progesterone levels in follicular fluid and serum of non pregnant one-humped she-camel in relation to follicular development

ABSTRACT

One hundred and thirty non pregnant one humped she-camel slaughtered in Cairo abbatoir were subjected for this present work. Blood samples were collected immediately after slaughtering. The sera were collected in clean dry vials. After evaceration the ovaries were collected from the corresponding animals. The diameter of the growing mature follicles was measured. According to the size of the follicles, the animals were classified into three groups Small [2-5 mm], medium [5-10 mm] and large sized follicles, [more than 10 mm]. The follicular fluid was aspirated from the follicles. In animals having small or medium follicles, the follicular fluid was pooled and considered as one sample. Finally ten pooled samples were obtained. Whereas animals with large size follicles, the follicular fluid was quite enough to fulfil all the required determinations. The collected sera samples of small and medium sized follicles were pooled to have ten corresponding pooled for each. The estradiol and progesterone concentration were determined in both serum and follicular fluid. The results revealed the following findings and conclusions: 1] serum estradial concentration showed a significant increase to reach its highest level in animals with large follicles. It was clearly obvious that, the hormone concentration in the follicular fluid is several times higher than in serum. 2] Serum progesterone concentrations of different groups were nearly similar to each other. Progesterone levels in the follicular fluid did not differ significantly. However, follicular progesterone concentration was also several times higher than that of serum