Dereplication of bioactive constituents of the genus hypericum using LC-[+,-]-ESI-MS and LC-PDA techniques: Hypericum triquterifolium as case study
SPJ-Saudi Pharmaceutical Journal. 2009; 17 (4): 277-285
in English
| IMEMR
| ID: emr-123255
ABSTRACT
Utilizing liquid chromatography-electro spray ionization-mass spectrometry [LC-[+,-]-ESI-MS] and liquid chromatography-photodiode array detection [LC-PDA] techniques, a dereplication strategy for the analysis of the secondary metabolites constituents of the genus Hypericum has been developed. From the crude methanolic extract of the aerial parts of H. triquetrifolium [leaves, stems, and flowers] and on the basis of their UV- profiles, chromatographic retention times and [+,-]-ESI-MS [TIC and SIM] mass spectral data, seven known [1-7] compounds were dereplicated fairly rapidly. The compounds were classified into three structural classes phloroglucinols hyperfirin and adhyperfirin; naphthodianthrones hypericin, pseudo-hypericin, proto-hypericin, and protopseudo-hypericin; and the flavonoid rutin
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Perylene
/
Phloroglucinol
/
Plants, Medicinal
/
Mass Spectrometry
/
Chromatography, Liquid
/
Clusiaceae
Language:
English
Journal:
Saudi Pharm. J.
Year:
2009
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