Comparative evaluation of jojoba oil versus formocresol pulpotomy in primary molars - in vitro study [histopathological and immunohistochemical]

ABSTRACT

This study was conducted to evaluate and compare the histopathological and immunohistochemical responses of dog's dental pulp to formocresol pulpotomy versus jojoba. It was conducted on 32 primary molars of four dogs between the ages of one to two months. One of the dogs was used as control, while the rest of the molars were treated by formocresol and jojoba oil [pulpotomies]. The animals were destroyed after one month. The pulp tissues of all the teeth were submitted to histological and immunohistochemical evaluation. The pulp of the Jojoba oil treated group revealed slight hypervascularization, some blood vessels showed dilatation and areas of pulp calcification were detected. The cellularity of the pulp and its fibrous elements appeared normally with no inflammatory cell infiltration, while the pulp treated with formocresol was highly vascularized with high number of chronic inflammatory cells, areas of calcification with obvious increased collagen fiber density, in addition to numerous vacuoles and necrotic areas. In addition jojoba oil showed moderate immunoreactivity of von Willebrand factor in endothelial cells which may indicate increase in nitric oxide synthase. Histological and immunohistochemical reactions of dental pulp to Jojoba oil were more favorable than formocresol. The greater success rate of jojoba oil rather than FC pulpotomy in this study is attributed to anti inflammatory effect of jojoba oil compared to formocresol