Comparison of several maturation including factors in dendritic cell differentiation
IJI-Iranian Journal of Immunology. 2010; 7 (2): 83-87
in English
| IMEMR
| ID: emr-123703
ABSTRACT
Dendritic cells [DCs] are professional antigen presenting cells that have an important role in the initiation of immune response. The use of maturation factors in dendritic cell differentiation provides a promising approach in immunotherapy. In this study, we compared tumor necrosis factor-alpha, polyribocytidylic acid, lipopolysacharide and CpG oligonucleotides in inducing dendritic cell maturation. We generated immature dendritic cells with GM-CSF in combination with IL-4 from peripheral blood mononuclear adherent cells and used tumor necrosis factor-alpha, polyribocytidylic acid, lipoplysacharide and CpG for the induction of dendritic cell maturation. CD83 maturation marker on the dendritic cells was analyzed by flowcytometry after 7 days. In addition, mixed leukocyte reaction between dendritic cells and T cells was performed by MTT proliferation assay. Flow cytometry results demonstrated a comparable high level of CD83 expression on the mature dendritic cells generated by TNF-alpha, CpG, Poly IC, and LPS treatment of the immature dendritic cells. However, a significantly poorer proliferation of lymphocytes cocultured with the Poly IC-treated DCs was observed compared to the CpG-treated DCs in mixed leukocyte reaction [p=0.025]. Our results indicated that all of studied maturation inducing factors can be used in DC maturation but TNF-alpha and CpG were the preferred in vitro maturation factors. It is concluded that maturation of dendritic cells by CpG motif and TNF-alpha can be used to regulate immune responses
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Oligodeoxyribonucleotides
/
Cell Differentiation
/
Lipopolysaccharides
/
Poly I-C
/
Tumor Necrosis Factor-alpha
Limits:
Humans
Language:
English
Journal:
Iran. J. Immunol.
Year:
2010
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