Conjucation of R-Phycoerythrin to a polyclonal antibody and F [ab'] 2 fragment of a polyclonal antibody by two different methods
AJMB-Avicenna Journal of Medical Biotechnology. 2010; 2 (2): 87-91
in English
| IMEMR
| ID: emr-123754
ABSTRACT
R-phycoerythrin [R-PE], a fluorescent protein from phycobiliprotein family, is isolated from red algae. Conjugation of antibodies to R-PE facilitates multiple fluorescent staining methods. In the present study polyclonal antibodies and polyclonal F[ab']2 fragment antibodies were conjugated to R-PE by two different methods. The efficiency of the methods was evaluated using Immunocytochemistry [ICC] and Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis [SDS-PAGE]. In the first conjugation method, PE was attached to SMCC linker followed by conjugation of antibody to PE-SMCC. In the second method, SH groups were added onto R-PE molecule, while the antibody was attached to SPDP linker. Then, the antibody-SPDP molecule was conjugated to R-PE. Our results showed that the two conjugation methods did not have any abrogative effects on the antibody binding activity
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Immunohistochemistry
/
Antibodies
Limits:
Animals
/
Humans
Language:
English
Journal:
Avicenna J. Med. Biotechnol.
Year:
2010
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