[ evaluation of melatonin effect on in-vitro development of mouse preimplantation embryos]

ABSTRACT

Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonm on in-vitro mouse preimplantation embryo development. Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-pentoneal injection of 5 lU/ml pregnant mares serum gonadotrophin and subsequent 5 ILJ/ml human chononic gonadotrophin [ip]. Embryos were cultured in T6 culture medium supplemented with different dosages of melatonin [0 [control group], 100x10[-6]M, 10 x10[-6]M, 1x10[-6], 100x10[-9]M and 10 x10[-9]M [1-5 treatment groups]]. With due attention to percent of embryos in different stages, the rate of development of embryos was assessed using of invert microscope. It is also compared to control group. The results showed that the rate of cleavage and development of mouse the embryos to blastocyst stage increased significantly in the development culture medium supplemented with 10 and 100 nM/mg of melatonin in comparison to control group [p< 0.001]. The results of this study demonstrate that, enriching the culture medium with melatonin, improve mouse preimplantation development