Histopathological and histochemical studies of the kidney after ligation of the ureter and uretero-cutaneous anastomosis

ABSTRACT

The present work was undertaken to study the histopathological of the ureter and ureterocutancous anastomosis. Twelve adult dogs were used in this work. They were classiffied into three equal groups and were designated as group A and B and C. Unilateral ligation of the ureters for one month was done at the proximal end in group A and at the distal end in group B and C. Thereafter in group C. the ureters were transected proximal to the previous ligature and anstomosed to the skin of the anterior abdominal wall. The animals were sacrificed one month after the operation. Samples were taken for histopathological and histoche-mical studies from the kidney of the operated side and the contralateral as well. Unilateral ureteral obstruction at the distal or the proximal and induced several histopathological changes in the kidney of the operated side. However, the contralateral kidney showed compensatory hypertrophy of most malpighian corpuscles and some degenerative changes in the renal tubules. The histopathological changes were found to be more marked in the kidney following proximal ureteral obstruction. The rekease of ureteral obstruction by the process of uretero-cutaneous anastomosis was followed by regeneration of many malpighian corpuscles and some glomeruli were hypertrophied. Decrease or absence of the alkaline phosphatase activity was noted in the malipighian corpuscles and the convoluted tubules of the kidney of the ligated side. However, the contralateral kidney after ureteral obstruction and the operated kidney following uretero-cutaneous anastomosis showed moderate activity for this enzyme in the malpighian corpuscles, distal convoluted tubules and loops of Henle while a strong activity was seen in the proximal convoluted tubules. The regenerating malpighian corpuscle are functionally active as indicated by their increased enzymatic activity