Antioxidant enzyme activities in hepatic tissue from children with chronic cholestatic liver disease

ABSTRACT

To study the oxidative stress status in children with cholestatic chronic liver disease by determining activities of glutathione peroxidase [GPx], superoxide dismutase [SOD] and catalase [CAT] in liver tissue. A total of 34 children suffering from cholestatic chronic liver disease were studied. They were selected from the Hepatology Clinic, Cairo University, and compared with seven children who happened to have incidental normal liver biopsy. The patients were divided into three groups extrahepatic biliary atresia [n=13], neonatal hepatitis [n=15] and paucity of intrahepatic bile ducts [n=6]; GPx, SOD and CAT levels were measured in fresh liver tissue using ELISA. In the cholestatic patients, a significant increase was found in mean levels of SOD, GPx and CAT in hepatic tissue compared to control children. The three enzymes significantly increased in the extrahepatic biliary atresia group, whereas in the groups of neonatal hepatitis and paucity of intrahepatic bile ducts, only GPx and CAT enzymes were significantly increased. Oxidative stress could play a role in the pathogenesis of cholestatic chronic liver diseases. These preliminary results are encouraging to conduct more extensive clinical studies using adjuvant antioxidant therapy