[Determination of numerical and structural chromosomal changes in skin cancer in rat and finding comparable part in human]

ABSTRACT

Rat and human skin tumors have high similarity in physiology and show comparable biochemical responsiveness. In this study we have recorded numerical and structural chromosomal changes in metaphases chromosomes in induced-breast cancer in rats. In this experimental study, a carcinogenic substance [7, 12-Dimethylbenz[alpha] anthracene, DMBA] [2.5 mg] was subcutaneously injected to SD rats. Since tumors were appeared in effected rats, they became subjects for histopathology and imunohistochemistry studies as well as cell culture, metaphase chromosomal preparations and finally G-banding staining. The data showed some un-randomly numerical and structural changes in different number of chromosomes. We found a gain in chromosomes number 8, 9, 10, 18 and a loss of numerical chromosomal changes in chromosomes number 4, 5, 6, 17 and marker chromosomes in diploid and triploid model of analyzed chromosomal sets. According to our reported chromosomal changes and also published articles, we predict that SRD5A2, BCAM, SIRT2, AKT2, MLANA, RHOB, CANX, TERT are probably involved in development of skin cancer. In addition, we recommend that the genes located in these chromosomal segments, which have been reported in these studies, should be subjected to more investigations