Green tea extract administration protects against the nephrotoxicity induced by gentamycin and amikacin in rats

ABSTRACT

Despite their beneficial effects, aminoglycosides including gentamycin [GEN] and amikacin [AK] have considerable nephrotoxic side effects. This study investigaged the effects of green tea [GT] extract on biochemical and morphological kidney damage induced by GEN and AK in rats. Sixty male albino rats were used in this study and divided into 6 groups each contains ten rats. The first group was the control group injected with 0.4 ml saline. Each rat of the second group was given 12.5 ml of green tea extract [3%] twice daily to drink it orally for 25 days. The 3[rd] group received GEN [80mg/kg] once daily intraperitoneally for 10 days. The 4[th] group was administered AK [180 mg/kg] once daily intraperitoneally for 10 days. The 5[th] group received GT extract for 15 days then concomitant with GEN for 10 days. The 6[th] group received GT extract for 15 days then concomitant with AK for 10 days. GEN and AK groups showed significant increase in serum urea and creatinine [Cr] which was significantly decreased in green tea consuming rats before GEN and AK administration. GEN and AK treated rats showed significant decrease in the activity of calalase enzyme and reduced glutathione level in kidney tissues which were significantly increased in GT consuming rats prior to GEN and AK injection. Light microscopic examination of kidney tissues of GEN and AK groups revealed tabular necrosis and degenerative changes which were modulated by the consumption of green tea prior to GEN and AK administration. In conclusion green tea ameliorates and modulates GEN and AK induced-nephrotoxicity and oxidative damage by enhancing the antioxidant defense system