protective effect of vitamin C and olive oil against the cardiotoxicity induced by cyclophosphamide in rats

ABSTRACT

This study was carried out to evaluate the protective role of Vit. C and olive oil against cyclophosphamide [CPA]-induced cardiotoxicity in rats. Sprague-Dawley rats weighing 220=30 g were divided into 6 groups, the 1[st] gp, served as control, the 2[nd] and 3[rd] grps., were treated orally, with 120mg/kg Vit.C or 1 ml/rat olive oil, respectively, the 4[th] gp. was injected with 150 mg/kg CPA, respectively. All treatments were done daily for 4 weeks. 24 hours after treatment, the animals were weighed, the blood was collected for biochemical analysis, then the animals were sacrificed and the heart was removed for reactive oxygen species [ROS], antioxidant detection, and histopathological examination. The obtained result showed that the treatment with CPA significantly increased [P<0.05] cholesterol, triglyceride [TG], LDH levels as well as relative weight ratio of heart and body weight when compared to control group. Administration of Vit. C or olive oil, significantly decreased [P<0.05] the levels of cholesterol, TG and LDH as well as heart body weight ration. ROS parameters showed a significant increase [P<0.05] in malondealhyde [MDA], and significant decrease [P<0.05] in glutathione [GSH] and superoxide dismutase [SOD] following CPA administration when compared with control. Administration of Vit.C or olive oil after CPA injection significantly decreased [P<0.05] the level of MDA and significantly increased [P<0.05] the levels of both GSH and SOD when compared with CPA group. The present data revealed that Vit. C and olive oil administration improved the heart histopathological changes in rats injected with CPA. These findings demonstrated that Vit.C and olive oil can be used as a potent free radical scavenger in animals to minimize the toxic effects of treatment with anticancer drugs