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Sero-diagnostic efficacy of antigens prepared from B. abortus and B.melitensis used for agglutination tests and IELISA
Veterinary Medical Journal. 2011; 59 (3): 219-223
in English | IMEMR | ID: emr-126590
ABSTRACT
In this study, 120 bovine and ovine serum samples were collected and tested for brucellosis with Rose Bengal test [RBT], modified Rose Bengal test [mRBT], buffered acidified plate test [BAPT] using conventional Rose Bengal antigen [RBA] and buffered acidified plate antigen [BAPA] and antigens prepared from B. melitensis [the main cause of brucellosis in Egypt] strain 16-M. Indirect ELISA was used for testing the same serum samples using 4 different coating antigens which were S-LPS and OMP antigens prepared from 16-M strain and S-LPS and OMP prepared from S19 strain. There were some differences between the results of conventional Rose Bengal and BAPA antigens prepared from B. melitensis 16-M strain. In the same time, there were no characteristic differences in results of Indirect ELISA
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Index: IMEMR (Eastern Mediterranean) Main subject: Agglutination Tests / Enzyme-Linked Immunosorbent Assay / Serologic Tests / Brucella melitensis Language: English Journal: Vet. Med. J. Year: 2011

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Index: IMEMR (Eastern Mediterranean) Main subject: Agglutination Tests / Enzyme-Linked Immunosorbent Assay / Serologic Tests / Brucella melitensis Language: English Journal: Vet. Med. J. Year: 2011