Silent effects of an antiperspirant containing aluminum chlorohydrate on cytogenetic, immunological and histomorphological profiles in rats

ABSTRACT

Twenty male rats were equally subdivided into control rats subjected to cutaneous rubbing of 1ml of distilled water daily on their shaved belly, and treated rats subjected to repeated cutaneous rubbing of 1ml of an antiperspirant 24 deodorant on their shaved belly, daily for 50 consecutive days. Blood samples were withdrawn from each rat at the end of exposure periods and equal aliquots of heparinized blood samples were prepared the first was used for total and differential leukocytic count and pahgocytic activity assay, while plasma of the second aliquot was utilized for screening concentrations of plasma total protein, albumin and globulin. Rats were then scarified, 2 hours after colchicines injection [i.p.], and their femoral bone marrows were flushed out for the preparation of metaphase flushed out for the preparation of metaphase plates and cytogenetic analysis of bone marrow chromosomes. The obtained data revealed a significant increase in the frequencies of aberrant cells and different types of chromosomal aberrations in the bone marrow cells of treated rats. Blood neutrophil phagocytic activities, plasma total protein and globulin were distinctly decreased and moderate histopathological and histomorphometric alterations of belly skin of the treated rats were observed. The study throws the light on the dangerous cumulative effects of this antiperspirant on cytogenetic, immunological and histomorphological profiles in Rats