Potential use of Biomphalaria alexandrina snail antigens for serodiagnosis of Schistosomiasis Mansoni by immunoblot analysis

ABSTRACT

The aim of this study was to evaluate the possible use of Biomphalaria alexandrina snail antigens in diagnosis of schistosomiasis mansoni using enzyme linked immunolectrotransfere blot [EITB]. S. mansoni adult worm crude antigens [AWA], feet and visceral humps of B. alexandrina and Bulinus truncatus were used. Hyperimmune mice sera [HIS] versus each antigen were prepared for diagnosis of S. mansoni using western blot [WB]. Snail foot antigens were more specific in antibodies detection than visceral hump antigens. Three of five polypeptides of B. alexandrina foot antigen identified by S. mansoni HIS showed specific positive reactivity. These polypeptides were at MW of 31/32 and 43 kDa. While, only one of the six polypeptides of B. alexandrina hepatopancrease antigen identified by S. mansoni HIS, at a MW of 43 kDa was specific. Similarly, 2 polypeptides at MW of 44 and 55 kDa were specific in detection of anti-S. haematobium antibodies. However, the antigenically active polypeptide of B. truncatus hepatopancrease antigen had no specific reactivity towards anti-S. haematobium antibodies. B. alexandrina foot antigens were the most specific of the tested snail antigens in diagnosis of schistosomiasis mansoni