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Two main tropane alkaloids variations of black henbane [hyoscyamus niger] under pgprs inoculation and water deficit stress induction at flowering stage
Journal of Medicinal Plants. 2013; 12 (45): 29-42
in English | IMEMR | ID: emr-126942
ABSTRACT
Plants infection with microorganisms as well as physical factors such as osmotic stresses especially drought stress induced particular secondary metabolite production. Plant root and shoot hyoscyamine [HYO] and scopolamine [SCO] variations were investigated under three water deficit stress [WDS] levels as 30 [W1], 60 [W2] and 90% [W3] water depletion of field capacity and Pseudomonas putida [PP] and P. fluoresence [PF] strains inoculation at flowering stage. Seedling radicles and culture media were inoculated with PP and PF [10[9] CFU/ml] strains. Monitoring of soil moisture levels was conducted using gravimetric method. Alkaloids extracted were identified by gas chromatography [GC] and gas chromatography-mass spectrometry [GC-MS] analysis. Results revealed that HYO was the prevalent alkaloid in root and shoot organs. The highest ratio of SCO to HYO [0.53] and the highest alkaloids both HYO and SCO content in root and shoot was observed in W3PF treatment. The largest total alkaloids [HYO+SCO] yield [25.7 mg.plant[-1]] was showed in PP treated plants under W1 conditions. Integrative use of effective Pseudomonades strains and WDS sounds to be an encouraging and eco-friendly strategy for increasing tropane alkaloids yield and content in Hyoscyamus niger root and shoot parts
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Index: IMEMR (Eastern Mediterranean) Main subject: Scopolamine / Tropanes / Water / Chromatography, Gas / Pseudomonas putida / Flowering Tops / Hyoscyamine / Gas Chromatography-Mass Spectrometry Language: English Journal: J. Med. Plants Year: 2013

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Index: IMEMR (Eastern Mediterranean) Main subject: Scopolamine / Tropanes / Water / Chromatography, Gas / Pseudomonas putida / Flowering Tops / Hyoscyamine / Gas Chromatography-Mass Spectrometry Language: English Journal: J. Med. Plants Year: 2013