[Application of L-arginine as nitric oxide inducer and indomethacin as prostaglandin inhibitor in Balb/c mice infected with leishmania major MRHO/IR/75/ER]

ABSTRACT

Leishmaniasis is one of the health problems in. This study is designed to inhibit PG production by Indomethacin and induce NO by L-Arginine precursor in L. major infected Balb/c mice. This was an experimental study. Animals, Male inbred Balb/c mice were used in this study. The total number of animals used in this experiment was 48 Balb/c mice, divided into 6 groups [n =8 mice/group] including Group 1 [naive], Group 2 [L. major + 0.4% Ethanol], Group 3 [L. major + lndomethacin], Group 4 [L. major + DW], Group 5 [L. major + L- Arginine] and Group 6 [L. major + Indomethacin + L-Arginine]. In addition to serum, liver and spleen suspensions were investigated for NO induction by using Griess microassay. The data was analyzed by Analysis of Variances [ANOVA] and Student's t-test using Graph Pad Prism Software. The results indicated that production of NO was inhibited in infected Balb/c mice by L. major as compared with naive animals [Group 1]. INDO as inhibitor PG [Group 2] showed their ability to elevate RNI levels in infect animals. INDO showed anti-leishmanial activity, as these compounds reduced the lesion sizes [P<0.001]. INDO as inhibitor PG [Group 3] showed ability to decrease PG levels in infect animals. Our data may indicate a possible role for L-Arg and INDO as novel drug targets for the treatment of cutaneous leishmaniasis in mouse model