Prevention and inhibition of TC-1 cell growth in tumor bearing mice by hpv16 e7 protein in fusion with shiga toxin B-subunit from Shigella dysenteriae
Cell Journal [Yakhteh]. 2013; 15 (2): 176-181
in English
| IMEMR
| ID: emr-127542
ABSTRACT
For immunotherapy of human papillomavirus [HPV] -16-associated cervical cancers the E7 protein is considered a prime candidate. However it is a poor inducer of cytotoxic T-cell response, when being used as a singular antigen in protein vaccination. Hence, in this study we focused on the utilization of a vaccine delivery system for prevention or treatment of cervical cancer. In this experimental study, we designed and evaluated a novel fusion protein comprising HPV16 E7 antigen fused to Shiga toxin B-subunit [STxB] as both an antigen vector and an adjuvant. Then we designed two preventive and therapeutic tumor models to investigate the prevention and inhibition of TC-1 cell growth in female C57BL/6 mice, respectively. In each model, mice were immunized with the recombinant protein of E7-STxB or E7 without any adjuvant. We demonstrated that prophylactic immunization of E7-STxB protected mice against TC-1 cells. Also in the therapeutic model, E7-STxB inhibited TC-1 tumor growth inlungs. The results were significant when compared with the immunization of E7 singularly. We concluded that immunization with the E7-STxB protein without any adjuvant could generate anti-tumor effect in mice challenged with TC-1 cells. This research verifies the clinical applications and the future prospects of developing HPV16 E7 therapeutic vaccines fused to immunoadjuvants
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Shigella dysenteriae
/
Uterine Cervical Neoplasms
/
Immunization
/
Shiga Toxin
/
Papillomavirus E7 Proteins
/
Mice
Limits:
Animals
Language:
English
Journal:
Cell J. [Yakhteh]
Year:
2013
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