[Evaluation of cells with CD 14 marker monocytes in patients with cutaneous leishmaniasis before and after medical treatment]

ABSTRACT

Leishmaniasis is one of the human diseases severity of wich varies from a self limiting cutaneous lesion to a severe visceral form. It is endemic in several parts of the world including our country Iran and especially in the province of Khorasan and the city of Mashhad. The study was intended to evaluate the percentage of monocellular macrophages in 36 patients and to investigate their role in prevention of the disease and its response to the treatment. The study was a case-control study conducted from August 2001 until October 2002 in Ghaem Hospital and Bu-Ali Research Ins. of Mashhad University of Medical Sciences and finally 29 patients were carried out the survey as the study group and 23 people of their families as control group. A blood sample of 10 cc was obtained from both groups before and after treatment. Compelet Blood Counting and CD14 marker studies by flow cytometry for evaluating monocyte percentage were used. Personal, laboratory and treatment data were recorded in a questionare. Qualitive and descriptive statistics were used for analysis. The percentage of monocytes in the whole population of patients was significantly more than control group [p=0.006].The percentage of monocytes in patients who gave a positive response to the treatment [group I] and those who did not recovered after the treatment [group 2] was significantly higher, with a p value of 0.0 13 and 0.015 respectively, comparing with the control group. In order to get a more delicate evaluation, the study was repeated again by flow cytomctry method considering the CD14 marker. The result was similar; percentage of CD14 was increased significantly in whole patients, first group and second group [p<0.001, p= 0.003 and p=0.015 respectively], comparing with the control group. Meanwhile, comparing the three studied groups before and after medical treatment, the percentage of monocytes slightly decreased; the study had a similar result conducted for evaluating CD14 marker. This study suggests the major role of monocytes in revealing the disease and perhaps decreased percentage of monocytes caused by the influence of medical treatment