In vitro immunomodulatory activity of psoralea corylifolia linn. seeds on human neutrophils

ABSTRACT

The seeds of Psoralea corylifolia [Leguminosae] were collected and crushed to obtain required particle size. Then the powder was subjected to exhaustive extraction with 95% alcohol. The alcoholic extract was fractionated into Petroleum ether [40-60°C], Chloroform and Ethyl acetate. The total alcoholic extract and each fraction were screened for in vitro immunomodulatory activity on human neutrophils using experimental models like Nitroblue tetrazolium qualitative assay, Granulocytes phagocytosis assay, Candidacidal assay and Chemotaxis assay. The total alcoholic extract has exhibited immunomodulatory activity in all parameters studied. It can be inferred from the study that the total alcoholic extract exhibited more prominent in vitro immunomodulatory activity in concentration of 50 mg/ml and 100 mg/ml