Formulation and HPTLC standardization of safoof-e-kasir-e-reeh, a unani formulation

ABSTRACT

Safoof-e-Kasir-e-Reeh is a Unani preparation containing Cumin [Zira safed], Ajowan [Ajwain], Black salt [Namak-e-Siyah], and Black Pepper [Filfll Siyah] used as carminative and antiflatulent. The Safoof [powder] was prepared by blending all the powdered ingredients after passing through 100 mesh sieve. The Safoof was extracted with methanol and filtered. The filtrate was used for estimation of piperine by High Performance Thin Layer Chromatographic technique using silica gel 60 F HPTLC plates [Merck]. Benzene Ethyl acetate Diethyl amine [603010] was used as mobile phase for piperine. The detection was performed at the wavelength of 254 nm. The piperine content was found to be 2.284%. This estimation technique is very much useful for the standardization of Unani formulations containing black pepper