Nephro-protective effect of a non-pharmacopoeal unani combination: an experimental study

ABSTRACT

Sufoof-e-Kulliya [SK] a non-pharmacopocul formulation, reported to be nephro-protectivc and useful in nephrotic syndrome like conditions, was studied for nephro-protective and steroidal activity in experimental models. Gentamicin [40 mg/kg IM] was administered in groups of albino rats twice a day for 7 days to produce the nephrotoxicity and the animals subjected to nephrotoxicity also received the different doses of SK, orally twice a day for 7 days. On the 8th day all the animals were sacrificed and blood samples were collected to estimate the serum urea and serum creatinine levels which were taken as the marker of renal function. In second test, the animals in different groups were administered with the test drug for 3 days while the animals in standard control group were given hydrocortisone in the dose of 200 micrograms by subcutaneous injection for the same period. On the fourth day all the animals were sacrificed and their thymus gland was dissected out. The body weight of thymus gland was recorded to assess the steroidal activity. The test drug was found to have significant nephro-protective and steroidal activity. In addition histopathology was also undertaken to estimate the noxious effect of Gentamicin on kidney and nephro-protective effect of test drug