Engipore acts on human bone marrow stem cells

ABSTRACT

Porous HA scaffolds are promising materials for tissue engineering because they offer a tridimensional support and serve as template for cell proliferation and at last tissue formation. Engipore provide a natural 3D scaffold with organic fibrous material in bone. However, how this material alters osteoblast activity to promote bone formation is poorly understood. To study how Engipore can induce osteoblast differentiation in mesenchymal stem cells, the expression levels of bone related genes and mesenchymal stem cells marker were analyzed. Engipore causes a significant induction of osteoblast transcriptional factors like SP7 and RUNX2 and of the bone-related gene osteocalcin [BGLAP]. The expression of CD105 was not significantly changed in stem cells treated with Engipore with respect to untreated cells, while SSP1 [osteopontin] was significantly down expressed thus reducing osteoclast activity. The obtained results can be relevant to better understand the molecular mechanism of bone regeneration