effects of Achillea wilhelmsii extract on rat's gastric motility at basal and vagal stimulated conditions

ABSTRACT

Achillea genius is widely used in traditional medicine for gastrointestinal disorders. The aim of this study was to investigate the effects of aqueous-ethanol extract of Achillea wilhelmsii on rat's gastric motility in basal and vagal stimulated conditions. Twenty four Wistar rats were randomly divided into two groups control and test. The extract was prepared by maceration which was used to prepare three 0.5 ml samples of three doses [0.5, 1 and 2 mg/kg] in the test group. The same volume of saline was used in the control group. Gastric motility was measured by inserting a small balloon in the stomach which was connected to a pressure transducer. The data were recorded for 25 min duration after each dose and these data were analyzed for 3 intermittent five min intervals [t[1] = 0-5, t[2]= 10-15 and t[3]= 20-25 min]. The extract at basal condition decreased intragastric pressure [IGP] by 1 mg/kg dose in the t[3] and 2mg/kg in the [t2] and t[3] intervals. The extract at vagal stimulated condition decreased IGP by 1 and 2mg/kg doses in the t[2] and t[3] intervals. The extract reduced contraction amplitude at basal condition by 2mg/kg dose in the t[2] and t[3] intervals. At vagal stimulated condition contraction amplitude was reduced by 1 mg/kg dose in the t[2] and t[3] by 2 mg/kg in all three intervals. The extract showed no effect on frequency of gastric contraction in either basal or vagal stimulated conditions. The extract showed an inhibitory effect on gastric motility in both basal and vagal stimulated condition. This inhibitory effect may be exerted by an antagonistic effect on acetylcholine dependent calcium influx or release of calcium from intracellular storage in gastric smooth muscle