[Correlation of serum levels of testosterone, and anatomical and histomorphometrical indices of the testis with lipids and lipoproteins profile in the mice]

ABSTRACT

The previous studies have suggested that dietary supplementation ingredients and its appropriate polyunsaturated fatty acids can increase spermatogenesis and improve sperm quality in the domestic mammals and the chickens. In this study, Correlation of serum levels of testosterone, and anatomical and histomorphometrical indices of the testis with lipids and lipoproteins profile in the mice were evaluated. In this experimental study, 60 adult male mice were randomly selected. After animal euthanasia, blood samples were collected by cardiac puncture. The serum concentrations of total cholesterol and triglyceride were measured by enzymatic method, lipoproteins were determined by precipitation method and testosterone was measured by radio immuno assay. Anatomical indices, including weight, scrotal circumference, length and width of the testis, were recorded. For histomorphometric study, the 5micro- sections were made and stained with Hematoxyline- Eosin. Data were analyzed by the Pearson correlation tests. The serum levels of HDL-c were significantly correlated with the diameter of the leydig cells, testicular tubular area, germinal epithelium height, scrotal circumference and as well as serum levels of testosterone. Serum triglyceride and VLDL-c values were inversely correlated with the diameter of the leydig cells and germinal epithelium height. Furthermore, the serum triglycerides levels had significant positive correlation with the testis weight. Based on this study, anatomical and histomorphometrical indices of the testis correlate positively with serum levels of HDL-c and also inversely correlate with the triglycerides and VLDL-c values in the male mice