Urine xanthine oxidase and myeloperoxidase activity in pediatric urinary tract infections

ABSTRACT

To investigate the relationships between urinary tract infection [UTI], and activity of antioxidant enzymes, xanthine oxidase [XO] and myeloperoxidase [MPO] in urine of children with UTI Prospective observational study. Department of Pediatrics, Kahramanrnaras Sutcu Imam University, Faculty of Medicine, Turkey. A total of 115 random children admitted to our hospital for urinary symptoms, 61 girls and 54 boys, aged between 2 and 15 years [average 10 years] were included. Study subjects were divided into four groups Group 1 consisted of 29 pyuria positive and urine culture negative children; Group 2 included 30 children with pyuria and positive urine culture; Group 3 included 26 pyuria negative and urine culture positive children and Group 4 included 30 with pyuria and negative urine culture. Measurement of urine XO and MPO activity were performed spectrophotometrically. Urine samples Antioxidant enzyme activity levels in urine were examined in children with urinary tract infection. Urinary XO activities in Group 2 were significantly higher compared with other groups [p < 0.001, p = 0.001 and p < 0.001, respectively]. Significantly different MPO activities were found between Group 1 and Group 2 [p = 0.007]. However, no Significant difference was found between Group 3 and 4. Significantly increased MPO and XO enzyme activity was found in children with UTI. Measurement of MPO and XO activity may be useful in children with urinary symptoms to diagnose UTI, before obtaining a positive urine culture