[Effects of long term perinatal administration of bupropion on population spike amplitude in neonatal rat hippocampal slices]

ABSTRACT

The perinatal effects of antidepressants on CNS due to its common usage are important issues in neuroscience research. Bupropion is an atypical antidepressant that is used in smoke cessation under FDA approve widely. The study of synaptic effects of bupropion can reveal its mechanism for nicotine dependence cessation. In this study the long term effects of perinatal bupropion on population spike [PS] amplitudes were investigated. The PS amplitude is a good parameter for synaptic plasticity. Hippocampal slices from 18-25 day old rat's pups were prepared. The exam groups included control and Bupropion treated groups. Bupropion [40 mg/Kg, i.p] was applied in perinatal period daily as pretreatment. Bupropion also was perfused in ACSF [10, 50, 200 micro mol, 30 minutes] and tested for PS amplitude. PS amplitude of Stratum Radiatum was measured before and after Bupropion perfusion. Amplitude of PS before Bupropion perfusion was fitted as 100% for baseline. A concentration of 10 micro M did not reduce PS amplitude and Bupropion had no significant effects on PS amplitude. Bupropion in concentration of 50 micro M could reduce the amplitude of responses in 50% of cases. The 200 micro M of Bupropion perfusion reduced population spike amplitude all slices [n=22]. In the last state population spike amplitude in 8 out of 22 slices completely abolished. Decrease population spike amplitude in non-treated slices with 200 micro M perfusion was more than treated slices. Analyzing of data showed that chronic perinatal exposure to Bupropion in concentrations 50, 200 micro M reduced PS amplitude and we found adaptation synaptic in perfusion 200 micro M compare with Bupropion slices with non-treated