effects of cholestasis and cirrhosis on gastric acid and pepsin secretions in rat: involvement of Nitric Oxide

ABSTRACT

The liver has major role in the organism homeostasis, interactions with other systems, synthesis and metabolism of bile production, drug detoxification and hormone inactivation. Cholestasis can be defined as an impairment of the bile flow which can lead to hepatocytes necrosis and finally cirrhosis. Some studies reported a gastric acid secretion reduction in cirrhotic subjects, while others reported normal production gastric acid secretion. Our aim was to evaluate the effects of cholestasis and cirrhosis on gastric acid and pepsin secretions and its possible mechanism in rat. Male Wistar rats were randomly divided into five groups [n=8] control, cholestasis, sham cholestasis, cirrhosis and sham cirrhosis. Laparotomy was done under general anesthesia and then bile duct ligation [BDL] was performed. After 2 and 4 weeks in cholestasis and cirrhosis groups respectively, gastric content was collected by wash-out technique. Basal and stimulated acid an pepsin secretions were measured by using titration and the Anson method respectively in all groups. In order to measure stimulated acid and pepsin secretions, pentagastrin [25 micro g/kg, i.p.] was used. Nitric Oxide [NO] metabolites of gastric tissue were determined by Griess microassy method. Acid and pepsin secretions were significantly reduced in cholestatic and cirrhotic rats in comparison with control and sham groups [P< 0.01]. NO metabolite of gastric tissue was significantly increased in cholestatic and cirrhotic rats [P> 0.01]. Reducing of gastric acid and pepsin output in cholestatic and cirrhotic rats may be due to increasing in NO content of gastric tissue