Immunogenicity of HLA-DR1 restricted peptides derived from leishmania major gp63 using FVB/N-DR1 transgenic mouse model
Iranian Journal of Parasitology. 2013; 8 (2): 273-279
in English
| IMEMR
| ID: emr-132847
ABSTRACT
Leishmaniasis is a worldwide disease prevalent in tropical and sub- tropical countries. In the present study the immunogenicity of three human HLA-DR1 restricted peptides derived from L. major gp63 protein was evaluated using FVB/N-DR1 transgenic mouse model. The immunity generated by three MHC class II - restricted peptides with the sequence of AARLVRLAAAGAAVT [AAR], AAPLVRLAAAGAAVT [AAP] and SRYDQLVTRVVTHE [ASR] derived from L. major gp63 protein were predicted using a web-based software [SYFPEITHI] and tested in FVB/N-DR1 transgenic mice. Immunization of FVB/N-DR1 transgenic mice with one of the three predicted peptides [AAR] resulted in high levels of Th1-type immune response as well as significant levels of IFN-gamma detected by Proliferation assay and ELISA. The results indicate a high level of immunogenicity for AAR, which can be a potent candidate for peptide vaccine in Leishmania infections.
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Peptides
/
Mice, Transgenic
/
HLA-DR1 Antigen
/
Immunogenetics
/
Mice
/
Antigens, Protozoan
Limits:
Animals
Language:
English
Journal:
Iran. J. Parasitol.
Year:
2013
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