Comparison of three immunodiagnostic tests in detection of trichinosis in naturally infected rats

ABSTRACT

The counter current immunoelectrophoresis [CEP], the enzyme linked immunosorbent assay [ELISA] and the indirect immunofluorescent [IF] were evaluated in detection of antibodies in naturally infected rats. The test subjects included 10 infected rats as indicated by digestion method of muscle biopsy and 13 rats free from trichinosis. Of the immunodiagnostic tests evaluated, the CEP gave the highest diagnostic accuracy. The ELISA was the least accurate. The high sensitivity and specificity of the CEP for detection of anti-trichinella antibodies suggested that it could be developed as a serodiagnostic test for trichinosis