[Assessment of bactaslyde media in diagnosis of salmonella in water and foods in comparison with standard method in order to use in crisis]

ABSTRACT

Salmonellas are responsible for more than 40 percent of foodborne diseases in the world. Today, rapid diagnostic methods are essential for detecting these organisms in food and water especially in crisis. The aim of this study was to assess the Bactaslyde media usability in detection of salmonella and compare it with standard culture method. In this laboratory study 20 samples of water and foods [pasteurized milk, water, raw Kabab and Raw Poultry Meat] were contaminated with salmonella standard strain [PTCC 1609] and then this salmonellas were detected by Bactaslyde culture media and standard culture method. Sensitivity, specificity, positive predictive value, negative predictive value and speed of two methods compared with each other. This study showed that Bactaslyde culture pouch number 4 can detect Salmonella in all 20 contaminated samples of food and water. The detecting time was 15 hours and 55 minutes in compare with 93 hours for standard method. Sensitivity of the test was 100%, specificity 90%, positive predictive value 90.91%, negative predictive value, 100% and validity of the test was 95%. It was found that Bactaslyde culture method can detect Salmonella as a rapid test in all food and water samples and it can be recommended for using in crisis. More research with more samples and different foods is recommended.