Dot-Elisa in diagnosis of schistosomiasis

ABSTRACT

1 ul of S. mansoni egg antigen was dotted directly on the nitrocellulose paper sheet acting as the adsorbent surface [9 dots/paper]. The sera of 25 Egyptian patients and 15 healthy persons [2 ul of each] were dotted over the antigen dots, then 2 ml of each of the blocking, washing, HRP-conjugated IgG and DAB adding procedures were added over the nitrocellulose paper in the Petri dish at room temperature. An intact brown circle [by naked-eye] indicated a positive in Dot-ELISA. There is an insignificant dot color intensities in different clinical stages of S. mansoni infected Egyptian, whereas a direct relation was obtained between egg count and color intensity of the dots. The test had 100% sensitivity and 86% specificity, thus it appears to be useful for both laboratory and field studies