[Effects of dietary gamma-linolenic acid and docosahexaenoic acid with paclitaxel on the treatment of mice mammary carcinoma]

ABSTRACT

Breast cancer is one of the most important causes of death in women. One of the various gene expression involved in breast cancer is human epidermal growth factor receptor 2 [HER2/neu] gene expression increases. Factors of dietary affect on regulation of hormone secretion and the rate of breast cancer. One of these factors is amount and type of fats in diet. Gamma-linolenic acid [GLA] and Docosahexaenoic acid [DHA] are members of poly unsaturated fatty acids. In this study, effects of dietary GLA and DHA alone or together with paclitaxel on treatment of mice mammary carcinoma has been evaluated. Thirty female balb/c mice were divided in six groups randomly. Carcinomatous mass induced by tumor implantation method. Spontaneous breast adenocarcinoma of mice were used as tumor stock. The tumors of these mice were removed aseptically, dissected into 0.5 cm3 pieces. These pieces were transplanted subcutaneously into their right flank. GLA and DHA added to the mice diet two week prior to tumor implantation. At the end of intervention, tumors were removed and HER2 gene expression was measured. The weight of animal and tumor volume measured weekly. It was not significant change in the weight of animals that consumed DHA and DHA with taxol. Tumor volume in those groups that received corn oil with taxol [P<0.01], DHA [P<0.05] and DHA with taxol [P<0.001] showed significant decrease in comparison with control group. HER2 gene expression in DHA with taxol decreased significantly in comparison with control group [P<0.05]. Consumption of DHA oil with taxol causes decrease the volume of carcinoma mass. The future studies with large number of sample is needed to support this finding.