[Effects of kerack used in addict Iranian people on fertility of adult mice]

ABSTRACT

Infertility is one of the most serious social problems. Illicit drug use can be an important cause of male factor infertility. Kerack which its use is rising up in Iran refers to a high purity street-level heroin [heroin Kerack]. Heroin Kerack used in Iran is an opioid and has harmful effects on body organs. The aim of this study is to investigate the effects of Kerack used in Iran on fertility adult mice. In this study, 25 male mice were divided into five groups [control, sham and three experimental]. Experimental groups of Kerack-dependent mice [received ascending dose of Kerack for seven days] were divided into three categories, experimental I, II and III. Experimental I was given Kerack at a dose of 5 mg/kg, experimental II 35 mg/kg and experimental III 70 mg/kg, intraperitoneally twice a day for a period of 35 days. The sham group received normal saline and lemon juice [2.6 micro l/ml] whilst the control group just received water and food. Mice were then scarified and sperm removed from cauda epididymis were analyzed for sperm count, motility, morphology [normal/abnormal] and viability. Testes were also removed, weighed and processed for light microscopic studies. The results showed that fertility were significantly decreased in addicted mice compared with control groups [P sperm parameters and thickness of seminiferous epithelium were significantly decreased in experimental groups [dosedependent] compared with sham and control groups [P dose Kerack injected [70 mg/kg] in comparison with control testes [P fertility for the first time. This effect is especially on epididymal sperm parameters in adult mice.