Development of a high-resolution melting method for screening R188h polymorphism in XRCC2 gene

ABSTRACT

The High Resolution Melting [HRM] method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Here, we developed a HRM method to screen R188H SNP [rs3218536] of XRCC2 and compared the results with a well known PCR-RFLP technique. Genomic DNA samples from 350 healthy individuals were obtained. PCR-HRM analysis and PCR-RFLP method were performed simultaneously. Three different melting profiles corresponding to three different genotypes recognized by HRM analysis. The results of PCR-RFLP showed no discrepancy. We concluded that the HRM technique can be used as a screening method for rapid discrimination of R188H genotypes in XRCC2 gene.