Biomarkers detection of basal cell carcinoma by proteomics analysis

ABSTRACT

Basal cell carcinoma [BCC] is a type of non-melanoma skin cancer that is the most common cancer type. So, in this study gene expression was detected in normal and cancer skin cells to find alteration gene expression to able help detection and care of this disease. Detection of this event is available by proteomics techniques. BCC and Normal tissues were taken from 3 patients and total protein was extracted by standard method of purification. The protein spots were separated by 2- dimensional electrophoresis [2DE], and the proteomes analyzed by other methods. Comparison of gene expression in normal and cancer cells was analyzed. Finally the desired proteins were identified using data banks. 87 protein spots were detected by 2DE gels with 76 spots having significantly different expression in normal and cancer gel images. 7 proteins were determined while comparing with informational banks. Results show the alteration of protein expression in cancer tissues; most of them decreased or even had no expression. These alterations in gene expression in both tissues can help to discover new biomarkers for detecting and curing of cancer