Determination of antigenic patterns of three taenia extracts and their use for differential diagnosis of taeniasis by immunoblot

ABSTRACT

Immunoserological differential diagnosis of Taenia spp. is the major contribution of the present work. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis [SDS-PAGE] was used to analyze the protein components of three Taenia extracts prepared from Cysticercus bovis, Taenia saginata and Taenia taeniaeformis. Antigenic components of the three taeniid antigens revealed by [SDS-PAGE] were [I] Cysticercus bovis cystic fluid antigen 180, 116, 90, 66, 45, 26, 17 and 10 kD. [II] Taenia saginata excretory secretory antigen [ES] 198, 180, 146, 116, 55, 45, 35 and 26 kD. [III] Taenia taeniaeformis whole crude antigen 170, 140, 120, 116, 66, 55, 45, 35, 26, 17 and 10 kD. The characterized extracts were used as antigens in Western Blot [WB] technique. Sera from immunized rabbits with Cysticercus bovis fluid antigens were used in detecting a variety of immunogenic bands [I] Cysticercus bovis 160, 116, 86, 52, 45, 38 and 29 kD. [II] Taenia saginata 86, 68 and 10 kD. [III] Taenia taeniaeformis 170, 116, 86, 68, 52, 45 and 29 kD. The present results suggested that the diagnostic bands by Western Blotare 160 and 38 kD for Cysticercus bovis, 10 kD for Taenia saginata [ES] antigen and 170 kD for Taenia taeniaeformis