Comparative study of the role of D- pencillamine and garlic extract in treatment of lead poisoning in adult albino rats

ABSTRACT

Lead is one of the most useful materials in the world. The aim of this study was to compare the curative role of D-penicillamine and garlic extract in treatment of lead poisoning in adult albino rats. The study included 80 adult albino rats consisted of 7 groups. The period of lead intoxication extended for 3 months followed by either 1 month treatment [penicillamine or garlic extract] or 1 month of no treatment. Group I consisted of 20 rats divided equally into the negative and positive control subgroups. Each of groups 11- VII consisted into 10 rats. Group [I [P] Pencillamine alone in a dose of 25 mg/kg 6 days/ week for 1 month. Group III [G] garlic extract alone in a dose of 20 mg/kg 6 days/ wk for I month. Group IV [Pb] lead poisoning in a dose of 20 mg/kg 6 days/ wk for 3 months. Group V [Pb-P] treatment with penicillamine in Pb poisoning. Group VI [Pb-G] treatment with garlic extract in Pb poisoning. Group VII [Pb-F] follow up of Pb poisoning without treatment. The results of this study revealed that lead poisoning significantly elevated blood lead level [BLL] and testicular lead level [TLL] when compared with the negative control rats. Lead was testicular toxicant as it produced atrophic germinal epithelium and arrested spermatogenesis. Treatment of Pb poisoning by penicillamine or garlic extract resulted in significant decrease in BLL and TLL with significant increase in urinary lead level [ULL] when compared with the Pb treated rats. Also, P and G treatment resulted in improvement of testicular structure. [n follow up group, BLL, ULL and TLL were significantly increased when compared with the negative control rats. Also, BLL was significantly decreased when compared with lead treated group. There was minimal improvement in testicular structure in Pb-F group. It was concluded that both penicillamine and garlic can treat lead poisoning. Moreover, garlic curative effect is better than that of penicillamine. garlic can be considered as a chelating agent in treatment of lead poisoning