[Effects of Scrophularia striata extract on human fibroblast cells]

ABSTRACT

Several studies have been performed on rapid wound healing. Because of minimal side effects of herbal drugs and role of firoblast cells in wound healing, in this study the effect of Scrophularia striata extract on human firoblast cell proliferation was evaluate. In this basic-applied study, Scrophularia striata seed extract was prepared by water based extraction methods. Foreskin fibroblast [Ffk] cells, maintaining in DMEM culture medium supplemented with 10% FBS in a humidified incubator [37°C and 5% CO2] were used. 100000 Ffk cells per well were exposed to different concentrations of Scrophularia striata extract [1, 3, 5, 7, 10 and 20 microg/ml] at 24, 48, 72, 96 hours. MTT assay was used to evaluate effects of extract on cell proliferation. S. Striata seed extract had stimulatory effects on human fibroblast cells proliferation. Incubation time was effective on cell proliferation, so that cell proliferation was diminished with increasing incubation time. This study showed that different doses of S. striata seed extract has various effects on improvement of wound healing and anti-tumor activity. Further investigations are recommended to detect optimum dose and mode of clinical applications