effect of tamoxifen on the fetal and neonatal ovarian follicles development in rats

ABSTRACT

The effect of tamoxifen as a selective estrogen receptor modulator which is widely used for treatment of early and metastatic breast cancer was investigated on the folliculogenesis in rat's fetuses and neonates. The pregnant rats assigned into test and control groups. Control group received olive oil and treatment groups received either 17-[3-estradiol [10 [micro g/kg/day] or tamoxifen [0.4 mg/kg/day] between days 8-13 of pregnancy. On day 20th of pregnancy the rats euthanized and the blood samples were collected for determination of FSH, E[2], and the fetuses fixed for histological studies. Another group of pregnant rats went forward to obtain their neonates and we euthanized the neonates and the genital system was collected for further histopathological analyses on day 5th. The histological examinations of the fetus's and neonate's ovaries and biochemical data showed significant changes in the rats which treated with tamoxifen. The absence of folliculogenesis and an increase in E[2] level in tamoxifen-treated group which accompanied with sharp decrease of FSH level in comparison with the control group were demonstrated. By contrary, E[2] treated group showed a positive progress in development in terms of the formation of secondary follicles and also supportive connective tissues in comparison with the control group. In conclusion, this study supports the previous findings showing that tamoxifen has effects on the development of ovaries and therefore, it should be avoided or used with great caution in pregnant women