[Determination of maximum transmission time of the labeled complex to malignant lymphatic cells using cell culture]

ABSTRACT

Nowadays, radiotherapy is considered as one of the main methods in cancer treatment, which uses radiation to destroy cancer cells while causing minimal damage to normal tissue and. Another cancer treatment method is photoelectron therapy, which is a new treatment for mass tumors. In this method, high atomic number drugs should be absorbed by tumor cells] while using carriers] so, their atomic number is increased compare to normal cells, which causes more damage to tumor cells when radiation is applied. Thus, applying accurate and effective photoelectron therapy would be possible. The purpose of this study was to identify and analyze the factors affecting uptake of Metrezoate labeled with gallium by lymphatic tumors in cell cultures. Since uptake of Gallium by Malignant lymphatic cells has been reported in numerous researches, Gallium was bonded with a contrast media [metrezoate]. Malignant lymphatic cells [Large Cell Lymphoma, Small cell Lymphoma and Bourkit Lymphoma] in specific colonies were grown in gel culture media. Metrezoate-gallium complex was added to the culture media in concentration of 50%, 60% and 75%. Then, absorbed volume was determined by the SPECT imaging system in 72 hrs post-addition of metrezoate-gallium complex and the cells count and the colonies area were measured. Obtained results in 72 hrs revealed that maximum absorption; count quantity and colonies area occurred 4 hrs post-addition of gallium-metrezoate complex solution. As results show, Gallium-metrezoate complex is significantly accumulated in malignant lymphatic cells. As effective atomic number of malignant lymphatic cells is enhanced, it is expected applying photoelectron therapy in this time leads to more effective treatment