Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and Metronidazole in their binary mixture
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (5): 865-871
in English
| IMEMR
| ID: emr-138403
ABSTRACT
Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline [DIHQ] and metronidazole [MTN]. In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform acetone glacial acetic acid [7.5 2.5 0.1, by volume] as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water methanol [6040, V/V, PH=3.6] as mobile phase at a flow rate of 0.7 mL.min[-1] and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Reference Standards
/
Solvents
/
Spectrophotometry, Ultraviolet
/
Buffers
/
Calibration
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Chromatography, Thin Layer
/
Iodoquinol
/
Technology, Pharmaceutical
Language:
English
Journal:
Pak. J. Pharm. Sci.
Year:
2013
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