RP-HPLC analytical method development and optimization for quantification of donepezil hydrochloride in orally disintegrating tablet
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (5): 961-966
in English
| IMEMR
| ID: emr-138416
ABSTRACT
An easy, fast and validated RV-HPLC method was invented to quantify donepezil hydrochloride in drug solution and orally disintegrating tablet. The separation was carried out using reversed phase C-18 column [Agilent Eclipse Plus C-18] with UV detection at 268 nm. Method optimization was tested using various composition of organic solvent. The mobile phase comprised of phosphate buffer [0.01M], methanol and acetonitrile [503020, v/v] adjusted to pH 2.7 with phosphoric acid [80%] was found as the optimum mobile phase. The method showed intraday precision and accuracy in the range of 0.24% to -1.83% and -1.83% to 1.99% respectively, while interday precision and accuracy ranged between 1.41% to 1.81% and 0.11% to 1.90% respectively. The standard calibration curve was linear from 0.125 micro g/mL to 16 micro g/mL, with correlation coefficient of 0.9997 +/- 0.00016. The drug solution was stable under room temperature at least for 6 hours. System suitability studies were done. The average plate count was > 2000, tailing factor <1, and capacity factor of 3.30. The retention time was 5.6 min. The HPLC method was used to assay donepezil hydrochloride in tablet and dissolution study of in-house manufactured donepezil orally disintegrating tablet and original Aricept
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Acetonitriles
/
Phosphoric Acids
/
Buffers
/
Calibration
/
Administration, Oral
/
Chromatography, High Pressure Liquid
/
Technology, Pharmaceutical
/
Methanol
/
Drug Stability
/
Chromatography, Reverse-Phase
Language:
English
Journal:
Pak. J. Pharm. Sci.
Year:
2013
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