Quality evaluation of Houttuynia cordata Thunb. by high performance liquid chromatography with photodiode-array detection [HPLC-DAD]

ABSTRACT

A new, validated method, developed for the simultaneous determination of 16 phenolics [chlorogenic acid, scopoletin, vitexin, rutin, afzelin, isoquercitrin, narirutin, kaempferitrin, quercitrin, quercetin, kaempferol, chrysosplenol D, vitexicarpin, 5-hydroxy-3,3',4',7-tetramethoxy flavonoids, 5-hydroxy-3,4',6,7-tetramethoxy flavonoids and kaempferol-3,7,4'-trimethyl ether] in Houttuynia cordata Thunb. was successfully applied to 35 batches of samples collected from different regions or at different times and their total antioxidant activities [TAAs] were investigated. The aim was to develop a quality control method to simultaneously determine the major active components in H. cordata. The HPLC-DAD method was performed using a reverse-phase C[18] column with a gradient elution system [acetonitrilemethanol-water] and simultaneous detection at 345 nm. Linear behaviors of method for all the analytes were observed with linear regression relationship [r[2]>0.999] at the concentration ranges investigated. The recoveries of the 16 phenolics ranged from 98.93% to 101.26%. The samples analyzed were differentiated and classified based on the contents of the 16 characteristic compounds and the TAA using hierarchical clustering analysis [HCA] and principal component analysis [PCA]. The results analyzed showed that similar chemical profiles and TAAs were divided into the same group. There was some evidence that active compounds, although they varied significantly, may possess uniform anti-oxidant activities and have potentially synergistic effects