Study of the ploidy and cellular cycle of hidra-denomas and hidradenocarcinomas: a series of 13 cases

ABSTRACT

To study by flow cytometry [FCM] the ploidy and the cellular cycle of nodular hidradenoma [NH] and hidradenocarcinoma [HC] and to assess the prognostic utility of this technique in such tumors. We studied retrospectively 2 HC and 11 NH one of which was considered as an atypical NH. Monoparametric study by FCM was realized on paraffin-embedded material. The extracted cells were marked by Propidium's lodure and cellular cycle was analyzed by the software Mod-Fit LT. Our study showed eleven 100% diploid profiles, 10 of which had low S-phase varying between 2 and 12%. All of these 11 tumors were NH. S-phase was high [23,79%] in a single case that corresponded to the atypical NH. Two tumors showed aneuploid profiles; these corresponded to the 2 HC. The results of the cytometrie study suit perfectly to those of the histopathologic examination. FCM could so help to establish the prognosis of these tumors. But further studies are necessary to determine the value of this technique