Efficient discrimination by MIRU-VNTRs of Mycobacterium tuberculosis clinical isolates belonging to the predominant SIT11/EAI3-IND ancestral genotypic lineage in Kerala, India

Biljo V., Joseph; Smitha, Soman; Veronique, Hill; R. Ajay, Kumar; Nalin, Rastogi; Sathish, Mundayoor

Biljo V., Joseph; Smitha, Soman; Veronique, Hill; R. Ajay, Kumar; Nalin, Rastogi; Sathish, Mundayoor.

International Journal of Mycobacteriology. 2013; 2 (4): 244-247

in English | IMEMR | ID: emr-140927

ABSTRACT

ABSTRACT

The present study evaluated the ability of MIRU-VNTRs to discriminate Mycobacterium tuberculosis [MTB] clinical isolates belonging to the SIT11/EAI3-IND ancestral genotypic lineage, which is highly prevalent in Kerala, India. Starting from 168 MTB clinical isolates, spoligotyping [discriminatory index of 0.9113] differentiated the strains into 68 distinct patterns, the biggest cluster being SIT11/48 SIT11 [n = 48]. The present study shows that 12-loci MIRUs and 3 ETRs allowed an efficient discrimination of these isolates [discriminatory indexes of 0.7819 and 0.5523, respectively]

Subject(s)

Humans; Minisatellite Repeats; Interspersed Repetitive Sequences; Genotype; Cell Lineage; Polymorphism, Restriction Fragment Length

Search on Google

XML

Index: IMEMR (Eastern Mediterranean) Main subject: Polymorphism, Restriction Fragment Length / Minisatellite Repeats / Cell Lineage / Interspersed Repetitive Sequences / Genotype Limits: Humans Language: English Journal: Int. J. Mycobacteriology Year: 2013

Similar

MEDLINE

LILACS

LIS

Search on Google

XML