Seed alkaloids content and antioxidant enzymes activity in black henbane as influenced by ammonium nitrate application and water deficit stress
Journal of Medicinal Plants. 2014; 13 (49): 75-86
in English
| IMEMR
| ID: emr-141789
ABSTRACT
Since alkaloids are nitrogenous compounds, the availability of nitrogen [N] is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. This study intended to investigate the nitrogen [N] fertilization and water deficit stress [WDS] effects on seed tropane alkaloids elicitation including hyoscyamine [HYO] and scopolamine [SCO], and also antioxidant enzymes activities variations including superoxide dismutase [SOD], peroxidase [POX] and catalase [CAT] in black henbane. Plants were treated with different nitrogen [0, 0.14, 0.28 and 0.56 g N pot[-1] as ammonium nitrate, N0-N3, respectively] and WDS treatments [30, 60 and 90% depletion of water from field capacity, W1-W3]. Alkaloids extracted were identified by gas chromatography [GC] and gas chromatography-mass spectrometry [GC-MS] analysis. Results showed that the highest alkaloid content values in seeds [HYO 0.145% dw; and SCO 0.271% dw] achieved in plants grown under sever [W3] and moderate [W2] WDS accompanied with nitrogen supply of 0.28 g N pot[-1] [N2], respectively. The maximum and minimum [2.112 and 0.114 g.plant[-1]] total alkaloids yield were obtained in N3W1 and N3W3 treatments, respectively. Furthermore, SOD activity increased with increasing N fertilization in all WDS treatments. CAT activity increased with WDS up to 60% FC, and then decreased with WDS increase. The POX activity was the opposite to that of CAT activity with N application under WDS levels. Our results suggest that N in appropriate level may act as a modifier for seed physiological responses and as an elicitor for tropane alkaloids biosynthesis pathway in black henbane [hyoscyamus niger] plants
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Index:
IMEMR (Eastern Mediterranean)
Main subject:
Seeds
/
Dehydration
/
Alkaloids
/
Nitrates
/
Nitrogen
/
Antioxidants
Language:
English
Journal:
J. Med. Plants
Year:
2014
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